Do you know these biosafety cabinet tips?2

About the placement of biological safety cabinets. Zhejiang net clean-up equipment staff said that from the front of the biological safety cabinet into the cabinet flow rate of about 0.45 m / s, according to the ideal layout, should be as far as possible in the safety cabinet on both sides of the back and left 30 cm The space, easy to maintain homework. In addition, the top of the cabinet also left a height of 30 ~ 35cm, so as to accurately measure the wind speed of the exhaust filter, leave enough space for its replacement.

About biological safety cabinet decontamination. In the decontamination process, the operator should pay special attention, because the dirt residue is likely to affect the results of the next experiment. For biosafety cabinet decontamination methods, the operator can place an appropriate amount of paraformaldehyde (the final concentration of paraformaldehyde in the air in the cabinet is 80%) on a hot plate or frying pan (outside the cabinet) Then place a bake pot or frying pan that contains 10% more POM than the paraformaldehyde (outside the cabinet). Note that the second container should be covered so that pre-neutralized paraformaldehyde can be minimized.

Without the front bulkhead, a plastic-type plastic bezel is required and glued to the front face with tape to ensure that no gas leaks into the room. After the paraformaldehyde in the pan has been completely volatilized or the switch has been on for 1 hour, the operator needs to turn the switch off, and then place the safety cabinet overnight. Next, the operator needs to remove the cover of the second plate and turn on the switch, when ammonia bicarbonate begins to evaporate. Finally, remove the front bulkhead (or plastic baffle) and the biological safety cabinet can be used.

About biological safety cabinet maintenance and repair. First, biological safety cabinets need regular cleaning and disinfection, cabinets and surface contaminants can be completed in the work and UV disinfection with 2% 84 disinfectant wipe. The outer surface of the cabinet can be cleaned daily with 1% of 84 disinfectant. Second, the inner surface of the safety cabinet should be decontaminated before and after each use, with a disinfectant to wipe the table surface and the inner wall, the use of disinfectant to kill all the microorganisms that may exist in the cabinet. Third, the use of biological safety cabinet after one year, companies should notify the manufacturer of the overall equipment maintenance, disinfection of the filter treatment, the maintenance record, in order to carry out the latter part of the maintenance and repair.

In fact, the above mentioned on the biological safety cabinet classification, selection, placement, decontamination and maintenance and repair are the most basic knowledge. However, although it is common knowledge for professionals, there are still many problems arising from various factors in the daily operations. Therefore, even if the operation of the equipment is simple and the routine maintenance is normal, operators and pharmaceutical companies should do a good job of ensuring the smooth running of the test.